Human SPARC Antibody Pair - BSA and Azide free (RMAB-0252184)
Cat. No.: RMAB-0252184
Category: Antibody Pair
INQUIRY
10 x 96 tests
The Antibody Pair can be used to quantify Human SPARC. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/mL as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results._x000D_
Product Features
| Conjugate | Unconjugated capture and detector antibodies |
|---|---|
| Species Reactivity | Human |
| Range | 500 pg/mL - 32000 pg/mL |
| Applications | Sandwich ELISA |
| Key Features | Unconjugated capture and detector antibodies; Adaptable to any antibody pair-based assay format; Antibody concentration ~ 1 mg/mL; BSA and azide free buffer - ready for conjugation; Reacts with: Human |
Target Information
| Target Symbol | SPARC |
|---|---|
| Target Name | SPARC |
| UniProt ID | P09486 |
| Cellular Localization | Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane. |
| Developmental Stage | Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair. |
| Function | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. |
| Sequence Similarities | Belongs to the SPARC family. Contains 1 EF-hand domain. Contains 1 follistatin-like domain. Contains 1 Kazal-like domain. |
Storage & Shipping
| Storage & Shipping | Store at 4°C. Please refer to protocols. |
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For research use only. Not for clinical use.
