Human/Mouse LEF1 Antibody Pair - BSA and Azide free (RMAB-0252026)
Cat. No.: RMAB-0252026
Category: Antibody Pair
INQUIRY
10 x 96 tests
Human/Mouse LEF1 Antibody Pair - BSA and Azide free is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify LEF1 in sandwich ELISAs and many other pair-based applications._x000D_
Product Features
| Conjugate | Unconjugated capture and detector antibodies |
|---|---|
| Species Reactivity | Mouse, Human |
| Range | 78 pg/mL - 5000 pg/mL |
| Applications | Sandwich ELISA |
| Key Features | Unconjugated capture and detector antibodies; Adaptable to any antibody pair-based assay format; Antibody concentration ~ 1 mg/mL; BSA and azide free buffer - ready for conjugation; Reacts with: Mouse, Human |
Target Information
| Target Symbol | LEF1 |
|---|---|
| Target Name | Lymphoid enhancer-binding factor 1 |
| UniProt ID | Q9UJU2 |
| Cellular Localization | Nucleus. Found in nuclear bodies upon PIASG binding. |
| Function | Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. |
| Domain | Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. |
| Sequence Similarities | Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain. |
Storage & Shipping
| Storage & Shipping | Store at 4°C. Please refer to protocols. |
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For research use only. Not for clinical use.
