iPSC-derived Mesenchymal Stem Cells; BYS0112 (RMCL-00260272)
Cat. No.: RMCL-00260272
Category: Primary cell
INQUIRY
1 vial
iPSC-derived Mesenchymal Stem Cells; BYS0112 are induced pluripotent stem cells that have been differentiated into multipotent stromal cells. iPSC-derived MSCs may be further differentiated into osteoblasts, chondrocytes, myocytes, and adipocytes. These cells can be used in bone cell lineage differentiation, regenerative medicine, cell therapy, exosome research, and cancer immunology research.
Product Features
| BSL | BSL-2 |
|---|---|
| Product Format | Frozen |
| Applications | High-throughput screening; Immunology; Stem cell research; Toxicology; Immuno-oncology; Cell and gene therapy (CGT) development |
| Specific Applications | Bone cell lineage differentiation, regenerative medicine, cell therapy, exosome research, cancer immunology. |
| Functional Test | Osteocyte, chondrocyte, and adipocyte differentiation; immunosuppression assay |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Morphology | Spindle-shaped, fibroblast-like |
QC
| Bacterial & Fungal Testing | Not detected |
|---|---|
| Mycoplasma Contamination | Not detected |
| Virus Testing | Hepatitis B virus (HBV): Not detected Hepatitis C virus (HCV): Not detected Human immunodeficiency virus 1 (HIV-1): Not detected Human immunodeficiency virus 2 (HIV-2): Not detected |
Doner Information
| Gender | Male |
|---|---|
| Ethnicity | White |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Reagents for Cryopreservation | We recommend cryopreserving this model in Stem Cell Freezing Media. |
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | Refer to the batch specific information for the total number of viable cells recovered from this lot of this product the total number of viable cells, determine how much surface area can be inoculated to achieve an initial seeding density of between 10,000 and 20,000 cells per cm2. Prepare the desired combination of flasks. Add 5 mL of complete growth media per 25 cm2 of surface area. Place the flasks in a 37°C, 5% CO2, humidified incubator and allow the media to pre-equilibrate to temperature and pH for 30 minutes prior to adding cells. While the culture flasks equilibrate, remove one vial of this product cells from storage and thaw the cells in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1 to 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point onward should be carried out under strict aseptic conditions. Transfer the vial content into 5mL of complete media taken in a 15 mL sterile conical tube – Centrifuge the tube at 270-300 xg for 5min. Aspirate supernatant and re-suspend the cell pellet in 5 mL of complete culture media. Take an aliquot for cell counting. seed 10,000 to 20,000 cells/cm2 to pre-equilibrated culture flasks prepared in steps 1 to 3 of Handling Procedure for Frozen Cells and Initiation of Culture. Cap and gently rock each flask to evenly distribute the cells. Place the seeded culture flasks in the incubator at 37°C with a 5% CO2 atmosphere. Incubate for at least 24 hours before processing the cells further. |
| Population Doubling Time | Approximately 32 to 35 hrs |
For research use only. Not for clinical use.
