L-WRN (RMCL-00260313)
Cat. No.: RMCL-00260313
Category: Cell Lines
INQUIRY
1 vial
L-WRN is a fibroblast-like cell that was isolated from the areolar of a male mouse. This cell line was deposited by T Stappenbeck.
Product Features
| BSL | BSL-2 |
|---|---|
| Product Format | Frozen |
| Applications | 3D cell culture |
| Specific Applications | This cell line is a source for producing Wnt-3A, R-spondin 3, and noggin conditioned medium which can be used for growing various mammalian tissue stem cells. |
| Product Type | Animal cells |
| Strain | C3H/An |
| Organism | Mus musculus, mouse |
| Tissue | Subcutaneous connective tissue; Adipose; Areolar |
| Morphology | fibroblast |
| STR Profiling | CO1 species: mouse |
| Genes Expressed | wnt-3A; R-spondin; noggin |
Doner Information
| Gender | Male |
|---|---|
| Age | 100 days |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Reagents for Cryopreservation | Complete growth medium supplemented with 20% (v/v) fetal bovine serum and 10% (v/v) DMSO |
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at 70°C. Storage at 70°C will result in loss of viability. Pre-warm growth medium (without G418 and without Hygromycin B) in flask at 37°C. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the Oring and cap out of the water. Thawing should be rapid (approximately 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. Transfer the vial contents to the culture pre-warmed flask with medium. Because cells are very fragile after thawing, centrifugation and excess pipetting must be avoided. Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet. After 24 hours, change medium with complete growth medium, which contains G418 and Hygromycin B. |
For research use only. Not for clinical use.
