Megakaryocyte Kit (ES or iPSC Differentiation) (RMMS-00560152)
Cat. No.: RMMS-00560152
Category: Specialized Medium Kits
Research Area: Cell Therapy Research, Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology
INQUIRY
1 Kit
For differentiation of human ES or iPS cells to megakaryocytes and platelets
Product Features
| Cell Type | Hematopoietic Cells, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Megakaryocytes |
|---|---|
| Formulation | Serum-Free |
| Applications | Differentiation |
| Description | Megakaryocyte Kit is designed for the serum-free and feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to polyploid megakaryocytes capable of shedding platelets and expressing CD41a and CD42b. This simple two-dimensional 17-day protocol includes two stages. During the first 12 days, Hematopoietic Supplement A and then Supplement MK1 are added to basal medium to induce cells toward megakaryocyte-biased hematopoietic progenitor cells. At the end of this stage, hematopoietic progenitor cells are easily harvested from the culture supernatant, and are further differentiated to mature megakaryocytes using Supplement MK2 and SFEM II for an additional 5 days. At the end of the protocol (Day 17), the cells are typically expanded 405-fold +/- 54-fold (95% CI), and on average 71% +/- 3. 3% (95 CI) of cells co-express CD41a and CD42b. Megakaryocyte Kit has been optimized for differentiation of ES/iPS cells maintained in, or-AOF. |
Storage & Shipping
| Storage | -20°C or colder |
|---|---|
| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.
