Neural Progenitor Cells Derived from Normal; Human (RMCL-00260292)
Cat. No.: RMCL-00260292
Category: Cell model
INQUIRY
1 vial
These normal neural progenitor cells are derived from Human [Non-Hispanic Caucasian Male] Induced Pluripotent Stem (IPS) Cells and can be used in neuronal differentiation and drug screening.
Product Features
| BSL | BSL-2 |
|---|---|
| Product Format | Frozen |
| Applications | Drug development; High-throughput screening; Neuroscience; Stem cell research; Toxicology |
| Specific Applications | Neuronal differentiation and drug screening |
| Functional Test | Exhibit differentiation potential of NPCs into Tuj1+ early neurons and TH+ dopaminergic neurons. |
| Product Type | Human cells |
| Tissue | Bone; Marrow |
| Morphology | Short spindle shape |
| Cell Type | Neural Progenitor Cell |
| Disease | Normal |
| STR Profiling | Amelogenin: X,Y CSF1PO: 12,13 D13S17: 10,12 D16S539: 8,11 D5S818: 10,13 D7S820: 8,9 TH01: 6,9.3 TPOX: 8,11 vWA: 16,17 |
| Antigen Expression | Nestin, Pax-6 |
QC
| Bacterial & Fungal Testing | Not detected |
|---|---|
| Virus Testing | Cytomegalovirus (CMV): Not detected Human papillomavirus (HPV): Not detected Human Immunodeficiency virus (HIV): Not detected Epstein-Barr virus (EBV): Not detected Hepatitis C virus (HCV): Not detected Hepatitis B virus (HBV): Not detected |
Doner Information
| Gender | Male |
|---|---|
| Age | 31 years |
| Ethnicity | White |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Reagents for Cryopreservation | We recommend cryopreserving this model in Stem Cell Freezing Media. |
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Handling procedure | Refer to the batch specific information for the total number of viable cells recovered from this lot of this product Lot is ~ 2. 8 x 106 Using the total number of viable cells, customers have to decide seeding for their experiments and applications. Prepare the desired combinations of culture dishes with required media. Place dishes in a 37°C, 5% CO2, humidified incubator and allow the media to pre-equilibrate to temperature and pH for 30 minutes prior to adding cells. While the culture dishes equilibrate, remove one vial of this product from storage and thaw the cells by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1 to 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point onward should be carried out under strict aseptic conditions. Add 4ml of base medium or complete growth media – into a sterile conical tube. Using a sterile pipette, transfer cells from the cryovial to the conical tube. Centrifuge at 200-300xg for 5min, remove supernatant and re suspend the pellet in complete growth medium Transfer cell suspension to each of the pre-equilibrated culture dishes in the required seeding density, gently rock each dishes to evenly distribute the cells. Place the seeded culture flasks in the incubator at 37°C with a 5% CO2 atmosphere. Incubate for at least 24 hours before processing the cells further. |
For research use only. Not for clinical use.
