Neural Progenitor Cells Derived from XCL-1 GFAPp-Nanoluc-Halotag (RMCL-00260303)
Cat. No.: RMCL-00260303
Category: Reporter-labeled cell; Cell model
INQUIRY
1 vial
Neural Progenitor Cells Derived from XCL-1 GFAPp-Nanoluc-Halotag is an astrocyte lineage reporter line that can be used in neuronal differentiation and drug screening.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | Assay development; Drug development; High-throughput screening; Neuroscience; Stem cell research; Toxicology |
| Specific Applications | Neuronal differentiation and drug screening; Quantitative measurement of astrocyte differentiation with Nano Luciferase expression under the control of the GFAP gene promoter. |
| Functional Test | Exhibit differentiation potential of NPCs into Tuj1+ early neurons and TH+ dopaminergic neurons. |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Tissue | Umbilical cord; CD34+ cord blood |
| Morphology | Short spindle shape |
| Cell Type | Neural Progenitor Cell |
| Disease | Normal |
| STR Profiling | Amelogenin: X,Y CSF1PO: 9,10 D13S17: 9,10 D16S539: 9,13 D5S818: 14,16 D7S820: 8,9 TH01: 9.3 TPOX: 8,11 |
| Antigen Expression | Nestin, Pax-6 |
QC
| Bacterial & Fungal Testing | Not detected |
|---|---|
| Virus Testing | Cytomegalovirus (CMV): Not detected Human papillomavirus (HPV): Not detected Human immunodeficiency virus 1 (HIV-1): Not detected Epstein-Barr virus (EBV): Not detected Hepatitis B virus (HBV): Not detected |
Doner Information
| Gender | Male |
|---|---|
| Age | Neonate |
| Ethnicity | White |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Handling procedure | Coat plates with Cell Basement Membrane and culture the NPCs with NPC Growth Medium. To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If, upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –80°C. Storage at –80°C will result in loss of viability. Preparation of Cell Basement Membrane coated plates: Thaw Cell Basement Membrane on ice or at 4°C Prepare a 150 µg/ mL working concentration of Cell Basement Membrane in cold DMEM: F-12 medium Add enough Cell Basement Membrane solution to cover the surface of the plate (e. g. 1 mL diluted Cell Basement Membrane /well of a 12-well plate) Incubate for 1 hour at 37°C prior to use Initiation of Cultures Prepare complete NPC growth medium following the instructions in the package and pre-warm that medium as well as DMEM: F12 in a 37°C water bath for 15-30 min. If using a small volume of medium (50 mL or less), warm only the volume needed in a sterile conical tube. Avoid warming complete medium multiple times. Obtain a 12-well plate with Cell Basement Membrane. Aspirate the Cell Basement Membrane medium and directly add 1. 5 mL of the complete NPC Growth Medium per well. Place the plate in the incubator for 15 minutes to allow the medium to reach its normal pH (7. 0-7. 6). Four to five wells of a 6-well plate may be needed for each vial of cells thawed. Transfer 9 mL of pre-warmed DMEM: F12 into a 15 mL conical tube for recovery of the NPCs from the frozen stock. Remove cryovial of frozen cells from liquid nitrogen storage. Thaw the cells by gently swirling in a 37°C water bath. To reduce the possibility of contamination, keep the cap out of the water. Thawing should be rapid (approximately 1 to 2 minutes). Remove the cryovial from water bath when only a few ice crystals are remaining. Sterilize the cryovial with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. Remove cells from the vial using a P1000 micropipette and transfer cells drop-wise into the 15 mL conical tube containing 9 mL DMEM: F12. Centrifuge cells at 270 x g for 5 minutes at room temperature. Aspirate the supernatant and discard. Gently tap the bottom of the tube to loosen the cell pellet. Add 4 mL of the complete NPC Growth Medium to the tube. Gently resuspend the pellet by pipetting up and down 3 or 4 times to make a single-cell suspension. Perform cell count by a Vi-Cell Analyzer or hemocytometer. |
| Population Doubling Capacity | ≥ 15 PDLs |
For research use only. Not for clinical use.
