Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric) (RMAB-0252303)
Cat. No.: RMAB-0252303
Category: Antibody Pair
INQUIRY
96 tests
The Overall Exosome Capture and Quantification Assay Kit (Previously known as ExoQuant Overall Exosome Capture and Quantification Assay Kit) is double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively)._x000D_
Product Features
| Species Reactivity | Human |
|---|---|
| Sample Type | Serum |
| Key Features | Assay type: Sandwich (quantitative); Detection method: Colorimetric; Platform: Pre-coated microplate (12 x 8 well strips); Sample type: Serum; Sensitivity: 0.039 ng |
Target Information
| Target Symbol | CD9 |
|---|---|
| Target Name | CD9 antigen |
| UniProt ID | P21926 |
| Cellular Localization | Membrane. |
| Function | Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. |
| Post-translational Modifications | Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. |
| Sequence Similarities | Belongs to the tetraspanin (TM4SF) family. |
Storage & Shipping
| Storage & Shipping | Store at 4°C. Please refer to protocols. |
|---|
For research use only. Not for clinical use.