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Overall Exosome Capture and Quantification Assay Kit

Overall Exosome Capture and Quantification Assay Kit (RMAB-0252302)

Cat. No.: RMAB-0252302

Category: Antibody Pair

INQUIRY 96 tests
The Overall Exosome Capture and Quantification Assay Kit (Previously known as ExoQuant  Overall Exosome Capture and Quantification AssayKit (Cell Media, Colorimetric) Kit), is a double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from cell culture supernatants. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively)

Product Features

Species Reactivity Human
Sample Type Cell culture supernatant
Key Features Assay type: Sandwich; Detection method: Colorimetric; Platform: Microplate (12 x 8 well strips); Sample type: Cell culture supernatant

Target Information

Target Symbol CD9
Target Name CD9 antigen
UniProt ID P21926
Cellular Localization Membrane.
Function Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Post-translational Modifications Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
Sequence Similarities Belongs to the tetraspanin (TM4SF) family.

Storage & Shipping

Storage & Shipping Store at 4°C. Please refer to protocols.

For research use only. Not for clinical use.