Anti-RUNX2 Monoclonal Antibody (RMAB-0251497)
Cat. No.: RMAB-0251497
Category: Primary Antibodies
Research Area: Cancer Research, Stem Cells, Epigenetics, Neuroscience
INQUIRY
100 μL
Customer Size
Anti-RUNX2 Monoclonal Antibody. Tested in IF, IHC, ICC applications. This antibody reacts with Human, Mouse, Rat.
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Form | Liquid |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Recombinant fragment: NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA, corresponding to amino acids 251-351 of Human RUNX2 with tag. |
| Applications | WB, IHC, IF, ICC, IP |
Target Information
| Target Symbol | RUNX2 |
|---|---|
| Target Name | Runt-related transcription factor 2 |
| UniProt ID | Q13950 |
| Function | Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. |
| Involvement in Disease | Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD); also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies. |
| Post-translational Modifications | Phosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-34. |
| Domain | A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites. |
| Sequence Similarities | Contains 1 Runt domain. |
Storage & Shipping
| Storage Buffer | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
|---|---|
| Storage & Shipping | Dry Ice |
For research use only. Not for clinical use.
