Bone Marrow-Derived Mesenchymal Stem Cells; Normal, Human (RMCL-00260222)
Cat. No.: RMCL-00260222
Category: Cell Lines
INQUIRY
1 vial
Bone marrow-derived mesenchymal stem cells are multipotent cells isolated from the bone marrow of a donor. The cells can be used in adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, and generation of iPS cell lines.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | Stem cell research; Cell and gene therapy (CGT) development |
| Specific Applications | Adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, creation of iPS cell lines |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Tissue | Bone |
| Morphology | Spindle-shaped, fibroblast-like |
| Cell Type | Mesenchymal Stem Cell |
| Disease | Normal |
| Specific Staining | Positive expression for CD29, CD44, CD73, CD90, CD105, and CD166 Negative expression for CD14, CD34, CD19, and CD45 |
QC
| Bacterial & Fungal Testing | Not detected |
|---|---|
| Mycoplasma Contamination | Not detected |
| Virus Testing | Human Immunodeficiency virus (HIV): Not detected Hepatitis C virus (HCV): Not detected Hepatitis B virus (HBV): Not detected |
Doner Information
| Gender | Lot-specific |
|---|---|
| Age | Adult |
| Ethnicity | Lot-specific |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Handling procedure | Refer to the Certificate of Analysis for the total number of viable cells recovered from this lot of this product. Using the total number of viable cells, determine how much surface area can be inoculated to achieve an initial seeding density of 5,000 cells per cm2. Prepare the desired combination of flasks. Add 5 mL of complete growth medium per 25 cm2 of surface area. Place the flasks in a 37°C, 5% CO2, humidified incubator and allow the media to pre-equilibrate to temperature and pH for 30 minutes prior to adding cells. While the culture flasks equilibrate, remove one vial of this product from storage and thaw the cells by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1 to 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point onward should be carried out under strict aseptic conditions. Add the appropriate volume of complete growth medium [volume = (1 mL x number of flasks to be seeded) -1 mL] into a sterile conical tube. Using a sterile pipette, transfer the cells from the cryovial to the conical tube. Gently pipette the cells to homogenize the suspension. Do not centrifuge. Transfer 1. 0 mL of the cell suspension to each of the pre-equilibrated culture flasks prepared in steps 1 to 3 of Handling Procedure for Frozen Cells and Initiation of Culture. Pipette several times, then cap and gently rock each flask to evenly distribute the cells. Place the seeded culture flasks in the incubator at 37°C, 5% CO2 atmosphere. Incubate for at least 24 hours before processing the cells further. |
| Population Doubling Capacity | ≥ 10 in complete growth medium and support differentiation |
For research use only. Not for clinical use.
