Feeder-free Medium (RMMS-00560238)
Cat. No.: RMMS-00560238
Category: Specialized Medium
Research Area: Stem Cell Biology
INQUIRY
500 mL
Serum-free medium for naïve-like human pluripotent stem cells
Product Features
| Overview | Revert primed human embryonic (ES) and induced pluripotent stem (iPS) cells to a naïve-like state and maintain cells in a naïve-like state under hypoxic conditions without bFGF or feeder cells. Developed under license from the Weizmann Institute of Science, this serum-free cell culture medium produces robust cultures with features of a naïve-like state, such as tightly packed, domed colonies with refractive edges. Human ES/iPS cells cultured in Feeder-Free Medium show increased expression of key transcripts associated with the naïve-like state, such as KLF17, KLF2, KLF4, and TFCP2L1, and can either be differentiated or converted back to a primed state by culture in and then differentiated. Compatible products that can be used for differentiation include Definitive Endoderm Kit, SMADi Neural Induction Kit, and Mesoderm Induction Medium. |
|---|---|
| Species | Human |
| Cell Type | Pluripotent Stem Cells |
| Applications | Cell Culture, Expansion, Maintenance |
| Formulation | Serum-Free |
Storage & Shipping
| Storage | -20°C or colder |
|---|---|
| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.
