PD0325901

PD0325901 is a selective, cell permeable inhibitor of the MEK/ERK pathway that inhibits the activation and downstream signaling of MEK. It is an extremely potent inhibitor, suppressing the phosphorylation of ERK in C26 cells at very low concentrations. Maintains undifferentiated mouse embryonic stem. Allows derivation and maintenance of rat ES cells. Add at the later stages of reprogramming to select for and expand fully reprogrammed mouse induced pluripotent. Increases the efficiency of reprogramming human somatic cells to iPS cells, in combination with SB431542 and Thiazovivin. Promotes reprogramming of human somatic cells to iPS cells using only a single factor, OCT4. Generates mouse-like or “ground state” iPS cells from human and rat somatic cells, in combination with CHIR99021 and A83-01.