Recombinant Human RSPO1 Protein (Active) (RMPP-00230932)
Cat. No.: RMPP-00230932
Category: Kinases
Research Area: Epigenetics and Nuclear Signaling
INQUIRY
25 μg
1 mg
Product Features
| Source | Baculovirus infected Sf9 cells |
|---|---|
| Purity | > 85% SDS-PAGE. The purity was determined to be >85% by densitometry. GST Affinity Column purified. |
| Nature | Recombinant |
| Animal Free | No |
| Form | Liquid |
| Applications | SDS-PAGE; WB; Functional Studies |
| Key Features | Expression system: Baculovirus infected Sf9 cells; Purity: > 85% SDS-PAGE; Active: Yes; Suitable for: SDS-PAGE, WB, Functional Studies |
Protein Information
| UniProt ID | Q9UBE8 |
|---|---|
| Molecular Weight | 84 kDa including tags |
| Sequence Similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. |
| Protein Length | Full length protein |
| Cellular Localization | Nucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic. |
| Function | Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3. |
| Post-translational Modifications | Dually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme. |
Storage & Shipping
| Shipping and Storage | Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. pH: 7.50Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride This product is an active protein and may elicit a biological response in vivo, handle with caution. |
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For research use only. Not for clinical use.
