Anti-RUNX2 Antibody (RMAB-0251015)
Cat. No.: RMAB-0251015
Category: Primary Antibodies
INQUIRY
100 μg
Customer Size
Mouse monoclonal to RUNX2
Product Features
| Isotype | IgG2a |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Clone Number | 2B9 |
| Form | Liquid |
| Purity | Tissue culture supernatant |
| Species Reactivity | Rat, Human, Recombinant fragment |
| Immunogen | Recombinant fragment: NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA, corresponding to amino acids 251-351 of Human RUNX2 with tag. |
| Applications | ICC/IF, Flow Cyt, WB |
| Key Features | Mouse monoclonal to RUNX2; Suitable for: ICC/IF, Flow Cyt, WB; Reacts with: Rat, Human, Recombinant fragment; Isotype: IgG2a |
Target Information
| Target Symbol | RUNX2 |
|---|---|
| Target Name | Runt-related transcription factor 2 |
| UniProt ID | Q13950 |
| Cellular Localization | Nucleus. |
| Function | Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. |
| Involvement in Disease | Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD); also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies. |
| Post-translational Modifications | Phosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-34. |
| Domain | A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites. |
| Sequence Similarities | Contains 1 Runt domain. |
Storage & Shipping
| Storage Buffer | pH: 7.40; Constituent: 2.68% PBS |
|---|---|
| Storage & Shipping | Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
