Anti-SMURF 2 Antibody (RMAB-0251068)
Cat. No.: RMAB-0251068
Category: Primary Antibodies
INQUIRY
100 μL
Customer Size
Rabbit monoclonal to SMURF 2
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | EP629Y3 |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide within Human SMURF 2 aa 2-3. The exact sequence is proprietary. |
| Applications | WB |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to SMURF 2; Suitable for: WB; Knockout validated; Reacts with: Human |
Target Information
| Target Symbol | SMURF2 |
|---|---|
| Target Name | E3 ubiquitin-protein ligase SMURF2 |
| UniProt ID | Q9HAU4 |
| Cellular Localization | Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. |
| Post-translational Modifications | Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1. |
| Domain | The second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain. |
| Sequence Similarities | Contains 1 C2 domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 3 WW domains. |
Storage & Shipping
| Storage Buffer | pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. |
For research use only. Not for clinical use.
